Astronaut in space

Martian greenhouse

Building a micro-ecosystem

Objective

Investigate the difficulties in building a stable ecosystem containing higher organisms, such as tomatoes and other green plants, in a relatively small space.

Some important concepts

An Ecosystem: An ecosystem is a community of living organisms interacting with each other and their non-living environment.

community + habitat = ecosystem

For this investigation we will provide a suitably prepared habitat within a sealed glass jar, into which we will place a small community of plants.

Dynamic Equilibrium

The micro-ecosystem in this project contains soil, an atmosphere, green plants, plus innumerable "stowaway" micro-organisms.

The only resource that enters and leaves the ecosystem is energy.

The energy balance is important to an ecosystem. When too much energy enters, the temperature will rise until the energy input is exactly balanced by the heat lost. Conversely, when the energy output (as heat) exceeds the energy input to the system, the temperature of the system will decrease until equilibrium is re-established.

Dynamic equilibrium

In order to achieve stability an ecosystem must attain a state of "dynamic equilibrium". In this state the (average) rate at which resources, such as carbon dioxide, are consumed, is balanced by the rate at which that resource is replaced through the process of recycling.

Dynamic equilibrium

A Stable Ecosystem

A stable ecosystem is one in which, on average, a state of dynamic equilibrium exists.

Environmental Conditions on Mars

Martian 'soil'

The Martian "soil" is about 40% SiO2 (silicon dioxide), a fine sand-like material and about 20% Fe2O3 (iron oxide) "dust". This dust is very fine, its texture is similar to that of talcum powder.

The remainder of the Martian soil consists of clays, dust, gravel, pebbles, stones and rocks of both simple and complex minerals similar to those found on Earth.

As far as we know, the Martian soil is sterile.

How fertile is the Martian soil? It's hard to say, but based on the results of both Viking landers and the recent Mars Pathfinder missions, the soil appears to be a much better medium for plant growth than most soils on the Earth, although Martian soils appear to be somewhat deficient in potassium.

Martian atmosphere

The atmospheric pressure on Earth is typically about 100kPa (kilopascals). On Mars it is less than 1kPa; far too low for either plants or humans.

Plants can survive with a mere 5 kPa atmosphere, 2kPa nitrogen, 2kPa oxygen, 0.6kPa water vapour, and less than 0.1kPa carbon dioxide and the remainder a mixture of gases such as argon and nitrogen; whereas, humans prefer at least 20kPa of oxygen and 10kpa nitrogen (as a buffer) to work and breathe comfortably (about 30kPa).

In a Martian greenhouse, astronauts will have to wear a space suit.

Temperature

On Mars, even in the equatorial zone, the temperature is perishingly cold; colder than anywhere on Earth, except perhaps during long polar winter nights near the South pole.

To grow plants on Mars a suitable warm environment will need to be created.

Martian greenhouse

Although plants can survive with less than 0.1kPa carbon dioxide they can survive with much more. In fact, most green plants prefer a carbon dioxide-rich atmosphere. On Mars, a greenhouse would not necessarily have to be a closed ecosystem. It is assumed that on Mars the atmospheric pressure and atmospheric composition within the greenhouse could be adjusted using outside resources. For example, excess methane gas could be vented outside of the greenhouse and perhaps more carbon dioxide could be added by extracting it from the Martian atmosphere, pressurizing it, and pumping it into the green house.

Similarly the amount of water and fertility of the soil could be adjusted using outside resources.

Student Activities: A Simulated Martian Greenhouse

Begin by reviewing the following steps, paying close attention to any special instructions and warnings.

Set up a journal in which to record all details related to the construction of your simulated Martian greenhouse and in which to record any observed changes that occur in your greenhouse from day to day and week to week.

Step 1

To build a simulated Martian greenhouse we need to find a large empty wide-mouth jar (with a lid), like the kind used by restaurants for Heinz ketchup and mustard.

Begin by preparing the soil. The bottom layer should consist of a thin layer of gravel covered with a layer 2-3cm thick of charcoal. These layers act as soil and air buffers to help reduce large swings in the moisture content, and chemical composition, of the atmosphere in your simulated Martian greenhouse.

For root support the top layer of the soil should consist of a layer of peat moss. (For a more realistic Martian soil-simulation, a mixture of sterile sand and clay can be substituted, but its water retention properties are much less than that of peat moss).

Finally, plant an assortment of small green plants, or alternately, plant a few tomato seedings.

Simulated martian greenhouse step 1

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Step 2

Once the plants are installed you may wish to wait a few days to allow the plant roots to establish themselves in their new environment before proceeding to this step.

Prepare the rim of the jar with a light coat of vacuum grease or with a strip of Teflon plumber's tape so that the lid can be installed immediately after the carbon dioxide has been poured into the jar.

To create a carbon dioxide atmosphere we will simply pour carbon dioxide (whose density is greater than that of air) into the jar.

A simple source of carbon dioxide can be obtained by reacting a generous quantity of ordinary baking soda (sodium bicarbonate) with a generous quantity of cold vinegar (diluted acetic acid) in a very large container. Allow the reaction to subside, then carefully pour the carbon dioxide (which is denser than air) into the greenhouse.

Simulated martian greenhouse step 2

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Step 3

The last step before screwing down the lid is to use a pair of tongs to insert a hot (120oC) bar of charcoal (which has been oven heated for at least one hour) into the jar. SEAL IMMEDIATELY!

Oven heating the bar of charcoal drives moisture and gases out of the bar. As the bar cools it will absorb an enormous quantity of carbon dioxide and will significantly reduce the gas pressure within the jar.

Simulated martian greenhouse step 3

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Step 4

The thin layer of vacuum grease (or Teflon ribbon) provides an airtight seal which preserves the low gas pressure within the jar, simulating a low pressure carbon dioxide Martian greenhouse atmosphere.

CAUTION: A sealed glass container should always be handled carefully.

Your simulated Martian greenhouse begins with a slight negative pressure of mostly CO2 but the pressure can drop dramatically because of carbon dioxide's very high solubility in water.

Water on the other hand evaporates very rapidly under low pressure conditions. If your jar is left in a sunny or hot environment the pressure inside can rise well above normal atmospheric pressure, resulting is an exploding jar!

Always wear eye protection and gloves when handling your micro-ecosystem.

Simulated martian greenhouse step 4

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Alternative to Step 2

If a compressed CO2 cylinder is available then CO2 can be added directly into the jar to displace the oxygen and nitrogen inside.

Simulated martian greenhouse step 2 alternate

Tips for a successful martian greenhouse

Plan an investigation

Experience has shown that in a sample of ten or twelve micro-ecosystems (a class set), some will survive only a few weeks, others will last a few months, and rarely, a few will survive more than a year.

The challenge is to determine, if possible, the reasons for the abrupt failure of some and the remarkable success of others.

A class discussion on this topic will elicit as many hypotheses explaining the failure/success of their greenhouse simulators as there are students. This provides an excellent opportunity to have students invoke the Scientific Method and to have them design further experiments to test their hypothesis.